abstract：:The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...

journal_title：Plant molecular biology

authors： Albani D,Parisi B,Carbonera D,Cella R

更新日期：1985-11-01 00:00:00

abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...

journal_title：Plant molecular biology

authors： Quigley F,Grienenberger JM,Weil JH

更新日期：1985-09-01 00:00:00

abstract：:Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...

journal_title：Plant molecular biology

authors： Altschuler M,Mascarenhas JP

更新日期：1985-09-01 00:00:00

abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

journal_title：Plant molecular biology

authors： Abbott AG,O'Dell M,Flavell RB

更新日期：1985-07-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：:Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...

journal_title：Plant molecular biology

authors： Helentjaris T,King G,Slocum M,Siedenstrang C,Wegman S

更新日期：1985-03-01 00:00:00

abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...

journal_title：Plant molecular biology

authors： Riccardi G,De Rossi E,Della Valle G,Ciferri O

更新日期：1985-03-01 00:00:00

abstract：:High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

journal_title：Plant molecular biology

authors： Wakarchuk DA,Hamilton RI

更新日期：1985-01-01 00:00:00

abstract：:A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...

journal_title：Plant molecular biology

authors： Fraley RT,Horsch RB,Matzke A,Chilton MD,Chilton WS,Sanders PR

更新日期：1984-11-01 00:00:00

abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

journal_title：Plant molecular biology

authors： Pfeiffer P,Laquel P,Hohn T

更新日期：1984-09-01 00:00:00

abstract：:The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

journal_title：Plant molecular biology

authors： Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

更新日期：1984-07-01 00:00:00

abstract：:Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determ...

journal_title：Plant molecular biology

authors： Hallick RB,Hollingsworth MJ,Nickoloff JA

更新日期：1984-05-01 00:00:00

abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

journal_title：Plant molecular biology

authors： van der Mark F,Klerk H,Bienfait F

更新日期：1983-11-01 00:00:00

abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...

journal_title：Plant molecular biology

authors： McNay JW,Pring DR,Lonsdale DM

更新日期：1983-07-01 00:00:00

abstract：:A mutant of alfalfa mosaic virus (AMV), which in contrast to wild type (wt) can invade cowpea plants systemically, is replicated more efficiently in cowpea protoplasts than the wt. Mutant preparations isolated from infected cowpea protoplasts contained a higher amount of middle component (M, containing RNA 2) than wt ...

journal_title：Plant molecular biology

authors： Roosien J,Sarachu AN,Alblas F,Van Vloten-Doting L

更新日期：1983-03-01 00:00:00

abstract：:Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...

journal_title：Plant molecular biology

authors： van Klaveren P,Slats J,Roosien J,van Vloten-Doting L

更新日期：1983-01-01 00:00:00

abstract：:Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

journal_title：Plant molecular biology

authors： Trapy G,Favre A,Esnault R

更新日期：1981-03-01 00:00:00